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SLE 331 Cellular Physiology: Melanophore Assignment T3 2013

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The aim of the assignment is to write a succinct report on your experiments with zebrafish melanophores performed in Practical 5. The assignment should include the following components: 1. A comparison of your findings to the average class data over previous years [on next page]. 2. A comparison of the melanophore control in zebrafish [based on your data and the class data] to published data for zebrafish found in the publication file on CloudDeakin. Your report should have the following elements. • A brief Introduction outlining the aim of practical 5. • A brief Methods section...

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Question Preview:

The aim of the assignment is to write a succinct report on your experiments with zebrafish melanophores performed in Practical 5. The assignment should include the following components: 1. A comparison of your findings to the average class data over previous years [on next page]. 2. A comparison of the melanophore control in zebrafish [based on your data and the class data] to published data for zebrafish found in the publication file on CloudDeakin. Your report should have the following elements. • A brief Introduction outlining the aim of practical 5. • A brief Methods section, summarised but not directly copied from the laboratory notes. • A summary of the Results that you obtained from each of your experiments. You can augment your written results with tabulation of data and diagrams if appropriate. Creative presentation of data is suggested. • A concluding Summary of your findings including the following elements: - An appropriate analysis that would lead the reader to understand what receptors and second messengers are used to control the melanophores of zebrafish, including what has been published for this species. - An analysis of the errors that may have occurred in your own experiments. Assessment of the assignment The primary criteria used to award marks for the assignment will be: - Your understanding of the experimental data and how it has been interpreted. - The interpretation of the data in the context of melanophore control in zebrafish. Melanophore assignment T3 2013 Melanophore Practical: Indicative class data from previous years

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We have seen various organisms which change their color as per the environment for example chameleons, fishes and various other amphibians. This is basically the cellular physiology assignment where we will study the experiment that was carried out on the Zebrafish Melanophores.

Aim of the Practical 5

  • To define the genes and proteins mediating the physiological pigmentation in Zebra fish.
  • To analyze the response of the pigmentation in Zebra fish and characterizing their functions accordingly.
  • Quantifying the response of fish on Melanin-concentrating and Melanocyte stimulating hormones.
  • Study molecular basis of pigment translocation.

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